Although antibiotics are considered one of the greatest discoveries of medical science, their misuse leads to antibiotic resistance, which is the most serious health problem of the 21st century. In short, the microorganisms causing the infection become resistant to the mechanism of action by using various methods. A better understanding of the bacteria-antibiotic relationship with multi-omics technologies has significant potential in developing treatment methods for antimicrobial resistance (AMR), investigating the effect of antibiotics on bacteria, and understanding the mechanism of resistance.
In the proposed project, an integrated proteomic and metabolomic study will be performed of the selected Escherichia coli (E. coli) strains with different resistance profiles and a reference K12 E.coli, active efflux pumps and multidrug resistance against some antibiotics (ampicillin, aztreonam, gentamicin and cefepime) that show resistance at different levels. With the information to be obtained from proteome and metabolome analyzes, identifying proteins and metabolites that play a role in antibiotic resistance and revealing their pathways will contribute to new antibiotic development studies. Genome sequencing information of these 3 special E. coli strains will also be obtained and used in analyzes to identify significant proteins. Within the scope of the project, the team consisting of project coordinator IY, MŞE, CP, GMK, EMA and graduate student KT will work together to complete the work packages. These work packages will last for a total of 24 months and the project activities will be carried out in Hacettepe University Advanced Technologies Application and Research Center, Department of Medical Microbiology, Bioinformatics Department and Kastamonu University, Biology Department, Microbiology Research Laboratory.